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2009•Molecular Cardiology of ChinaRequires access

In vitro culture of late-outgrowth endothelial progenitor cells from rat peripheral blood

Zhao Ji

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Abstract

Objective To evaluate the characterization of endothelial progenitor cells(EPCs)from rat peripheral blood cultured in vitro.Methods 10ml peripheral blood was obtained from the abdominal aorta.Mononuclear cells were isolated by using Ficoll density gradient centrifugation method,and cultured in EGM-2 medium.Result Late-EPCs could be obtained after 10 days.EPCs were able to line up in the cord-like structure and form clusters.Cells showed CD34、CD133、CD31、FLk-1、vWF、UEA-1/acLDL positive.,and high expressed CD34、CD133、FLk-1、eNOS mRNA.Irregular cells couldn't express corresponding markers.Conclusion Late-outgrowth endothelial progenitor cells could be obtained from rat peripheral blood through long-term in vitro culture.The cells show the characterization of endothelial cells.

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Objective To evaluate the characterization of endothelial progenitor cells(EPCs)from rat peripheral blood cultured in vitro.Methods 10ml peripheral blood was obtained from the abdominal aorta.Mononuclear cells were isolated by using Ficoll density gradient centrifugation method,and cultured in EGM-2 medium.Result Late-EPCs could be obtained after 10 days.EPCs were able to line up in the cord-like structure and form clusters.Cells showed CD34、CD133、CD31、FLk-1、vWF、UEA-1/acLDL positive.,and high expressed CD34、CD133、FLk-1、eNOS mRNA.Irregular cells couldn't express corresponding markers.Conclusion Late-outgrowth endothelial progenitor cells could be obtained from rat peripheral blood through long-term in vitro culture.The cells show the characterization of endothelial cells.

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Available abstract

Objective To evaluate the characterization of endothelial progenitor cells(EPCs)from rat peripheral blood cultured in vitro.Methods 10ml peripheral blood was obtained from the abdominal aorta.Mononuclear cells were isolated by using Ficoll density gradient centrifugation method,and cultured in EGM-2 medium.Result Late-EPCs could be obtained after 10 days.EPCs were able to line up in the cord-like structure and form clusters.Cells showed CD34、CD133、CD31、FLk-1、vWF、UEA-1/acLDL positive.,and high expressed CD34、CD133、FLk-1、eNOS mRNA.Irregular cells couldn't express corresponding markers.Conclusion Late-outgrowth endothelial progenitor cells could be obtained from rat peripheral blood through long-term in vitro culture.The cells show the characterization of endothelial cells.

Key concepts: Ficoll, Progenitor cell, CD34, Peripheral blood mononuclear cell, CD31, Cord blood, In vitro, Differential centrifugation

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