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Research on Free Sugars of Juice By HPLC-ELSD

SU Xiao-jian

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Abstract

Using HPLC method to determine the free sugars in watermelon juice.The optimum condition was as follows:Chromatographic column CLC-NH2(M),flow rate 1.0 mL/min,column under the condition of room temperature and the mobile phase consisted of an aqueous solution of acetonitrile-ultra pure water(80∶20).The results showed that this method expressing high average recoveries ranged from 97.88% to 99.35% and good precision ranged from 0.79% to 3.62%.Free sugars in juice can be determined rapidly and effectively in this way.

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What this paper is about

Using HPLC method to determine the free sugars in watermelon juice.The optimum condition was as follows:Chromatographic column CLC-NH2(M),flow rate 1.0 mL/min,column under the condition of room temperature and the mobile phase consisted of an aqueous solution of acetonitrile-ultra pure water(80∶20).The results showed that this method expressing high average recoveries ranged from 97.88% to 99.35% and good precision ranged from 0.79% to 3.62%.Free sugars in juice can be determined rapidly and effectively in this way.

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Available abstract

Using HPLC method to determine the free sugars in watermelon juice.The optimum condition was as follows:Chromatographic column CLC-NH2(M),flow rate 1.0 mL/min,column under the condition of room temperature and the mobile phase consisted of an aqueous solution of acetonitrile-ultra pure water(80∶20).The results showed that this method expressing high average recoveries ranged from 97.88% to 99.35% and good precision ranged from 0.79% to 3.62%.Free sugars in juice can be determined rapidly and effectively in this way.

Key concepts: Chromatography, Chemistry, High-performance liquid chromatography, Chromatography detector, Aqueous solution, Free water, Acetonitrile, Volumetric flow rate

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