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HPLC Determination of Sugars and Starch in Green Beans

Julia López-Hernández, María-José González-Castro, Manuel Vázquez-Blanco, Ma Lourdes Vázquez‐Odériz, J. Simal‐Lozano

Open publisher page 21 citations

Abstract

ABSTRACT A reversed‐phase HPLC method using a Spherisorb NH 2 column and 85:15 v/v acetonitrile/water as mobile phase was developed for determination of starch and soluble sugars (glucose, fructose and sucrose) in plants. When applied to pure starch samples, the analytical method was as accurate as the official AOAC method and had better precision (coefficient of variation 0.87% vs 1.27%). When applied to green bean ( Phaseolus vulgaris L.), recovery was 97.7% for starch, 98.0% for glucose and 98.1% for both fructose and sucrose.

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What this paper is about

ABSTRACT A reversed‐phase HPLC method using a Spherisorb NH 2 column and 85:15 v/v acetonitrile/water as mobile phase was developed for determination of starch and soluble sugars (glucose, fructose and sucrose) in plants. When applied to pure starch samples, the analytical method was as accurate as the official AOAC method and had better precision (coefficient of variation 0.87% vs 1.27%). When applied to green bean ( Phaseolus vulgaris L.), recovery was 97.7% for starch, 98.0% for glucose and 98.1% for both fructose and sucrose.

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Available abstract

ABSTRACT A reversed‐phase HPLC method using a Spherisorb NH 2 column and 85:15 v/v acetonitrile/water as mobile phase was developed for determination of starch and soluble sugars (glucose, fructose and sucrose) in plants. When applied to pure starch samples, the analytical method was as accurate as the official AOAC method and had better precision (coefficient of variation 0.87% vs 1.27%). When applied to green bean ( Phaseolus vulgaris L.), recovery was 97.7% for starch, 98.0% for glucose and 98.1% for both fructose and sucrose.

Key concepts: Fructose, Sucrose, Phaseolus, Starch, Chemistry, Chromatography, High-performance liquid chromatography, Carbohydrate

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