Genetic Analysis of a Rolled Leaf Mutation by T-DNA(Ds) Insertion in Oryza sativa

A rolled leaf mutant,Oryza sativa L.subsp.japonica IL39,caused by T-DNA(Ds) insertion was identified.Genetic analysis of the mutant showed that the two types of phenotype,rolled leaf and normal leaf in the segregating populations derived from the T-DNA(Ds) heterozygotes,fit the ratio of(3∶1.) Test for Basta resistance showed that the rolled leaf plants were all resistant while the normal leaf plants were susceptible.The ratio of resistant and susceptible plants is 3∶1,which indicate that the rolled leaf mutant is co-segregated with Basta resistance.The rolled leaf mutant caused by T-DNA(Ds) insertion was further confirmed by T-DNA detection using PCR method.This rolled leaf mutant is useful for isolation of the tagged gene in rice.