Effect of Lipofectamine-Mediated Small Hairpin Interference RNA on Expression of Excision Repair Cross-Complementing Group 1 in Cisplatin Resistant Ovarian Cancer Cell Lines

Objective: To investigate the interfering effect of lipofectamine-mediated small hairpin interfering RNA (shRNA) on expression of the excision repair cross-complementing group 1(ERCC1) gene in ovarian cancer cell lines. Methods: The small hairpin interfering RNA targeting ERCC1 gene(ERCC1-shRNA) was synthesized and designed. Recombinant plasmid expression vector(PERCC1-shRNA) which carrying ERCC1-shRNA was constructed and transfected to COC1/DDP cells with Lipofectamine 2000. RT-PCR and Western blot analysis were used to detect the expression of ERCC1 mRNA and protein after transfection. Flow cytometry was used to detect cell cycle and apoptosis. Results: RT-PCR analysis showed that the expression of ERCC1 mRNA and protein were significantly down-regulated after transfection(F=203.73,P 0.01).The apoptosis rate was significantly raised after 48 hours of transfection(t=20.65,P 0.01). Conclusion: The recombinant plasmid expression vector which carrying ERCC1-shRNA may remarkably down-regulate the expression of ERCC1 mRNA and protein after transfection,and induce the apoptosis in COC1/DDP cells.