2009•Tianjin yiyaoRequires access
Silence Effect of Short Hairpin RNA on Expression of PIM-1 in Prostate Cancer Cell Lines
Changyi Quan
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Abstract
Objective:To investigate the interference effect of lipofectamine-mediated short hairpin RNA(shRNA) on the expression of PIM-1 gene in prostate cancer cell lines.Methods:Three kinds of recombinant plasmid expression vectors(PPIM1-shRNA-1,-2,-3) were constructed,which can produce shRNA targeting different sequence of PIM-1 mRNA.The recombinant plasmid vectors were transfected into PC-3 cell lines,respectively.RT-PCR and Western blot assay were used to detect the expression of PIM-1 mRNA and protein after transfection.Results:The expression of mRNA and protein of PIM-1 in PC-3 were inhibited significantly at 48h post-transfection.Furthermore,PPIM1-shRNA-2 and PPIM1-shRNA-3 resulted in a stronger silencing effect than that of PPIM1-shRNA-1.Conclusion:The recombinant plasmid expression vector which carrying PIM-1 shRNA may remarkably inhibit the expression of PIM-1 mRNA and protein in PC-3 cell lines.The experiment provided the basis for further investigation of the role of PIM-1.