Study on Solubility Improvement of Rice Protein
Huang Jian-sha
Abstract
Huang Jian-sha
Abstract
[Objective]In order to improve the solubility of rice protein,hydrolysis process for rice protein with Alcalase was studied. [Method]The effects of the main parameters including pH,hydrolysis temperature,enzyme concentration,substrate concentration,hydrolysis time on hydrolysis reaction were investigated. The optimum conditions had been found by single-factor analysis and orthogonal test. [Result] The optimum conditions were showed as following,pH was 8. 0,hydrolysing temperature was 55 ℃,enzyme concentration of substrate was 1 200 U /g,substrate concentration was 4%,hydrolysis time was 2 h,solubility of rice protein was 71. 25%. [Conclusion]After enzymatic hydrolysis,the solubility of rice protein increased significantly.
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[Objective]In order to improve the solubility of rice protein,hydrolysis process for rice protein with Alcalase was studied. [Method]The effects of the main parameters including pH,hydrolysis temperature,enzyme concentration,substrate concentration,hydrolysis time on hydrolysis reaction were investigated. The optimum conditions had been found by single-factor analysis and orthogonal test. [Result] The optimum conditions were showed as following,pH was 8. 0,hydrolysing temperature was 55 ℃,enzyme concentration of substrate was 1 200 U /g,substrate concentration was 4%,hydrolysis time was 2 h,solubility of rice protein was 71. 25%. [Conclusion]After enzymatic hydrolysis,the solubility of rice protein increased significantly.
Key concepts: Hydrolysis, Solubility, Substrate (aquarium), Chemistry, Enzyme, Rice protein, Chromatography, Enzymatic hydrolysis