Optimization of extraction process for Lycii Fructus total flavonoids by central composite design-response surface methodology

Objective: To optimize the extraction process of Lycii Fructus total flavonoids by central composite design-response surface method. Method: Methanol concentration, extraction time and liquid-solid ratio were used as independent variables, and the contents of Lycii Fructus total flavonoids were used as dependent variables. The effect of three independent variables on the content of Lycii Fructus total flavonoids was investigated by multiple linear regression and binomial fitting using Box-Behnken design model. Result: The optimization extraction process of total flavonoids was 100% methanol, reflux extraction time 90 min with 60 fold of solvent. Conclusion: Central composite design-response surface method can be used to optimize extraction of Lycii Fructus total flavonoids, and the optimum mathematic model is highly predictive and the experimental design method is reliable.