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Segregation of RAPD markers: A case in interspecific cross of Eucalyptus

Siming Gan, Jisen Shi, Jiayu Bai, Kunming Wu, Juying Wu

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Abstract

Seven 10-mer primers were used for analysis on segregation of RAPD (Random amplified polymorphic DNA) markers in an interspecific F_(1) population of Eucalyptus urophyllaXE. tereticornis, including 2 parents and 212 sibs. Of the total 40 fragments amplified, 28 (70%) were polymorphic between parents and 24 (60%) segregant in the sibs, indicating a high level of polymorphism and heterozygosity at RAPD loci between the two parents. Those markers that were polymorphic between parents and segregating 1:1 in the sibs, following a pseudo-testcross configuration, could be potentially useful for genetic linkage map construction of the representative species, which would lay a theoretical basis for genetic linkage map construction using RAPD markers and F_(1) population.

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What this paper is about

Seven 10-mer primers were used for analysis on segregation of RAPD (Random amplified polymorphic DNA) markers in an interspecific F_(1) population of Eucalyptus urophyllaXE. tereticornis, including 2 parents and 212 sibs. Of the total 40 fragments amplified, 28 (70%) were polymorphic between parents and 24 (60%) segregant in the sibs, indicating a high level of polymorphism and heterozygosity at RAPD loci between the two parents. Those markers that were polymorphic between parents and segregating 1:1 in the sibs, following a pseudo-testcross configuration, could be potentially useful for genetic linkage map construction of the representative species, which would lay a theoretical basis for genetic linkage map construction using RAPD markers and F_(1) population.

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Available abstract

Seven 10-mer primers were used for analysis on segregation of RAPD (Random amplified polymorphic DNA) markers in an interspecific F_(1) population of Eucalyptus urophyllaXE. tereticornis, including 2 parents and 212 sibs. Of the total 40 fragments amplified, 28 (70%) were polymorphic between parents and 24 (60%) segregant in the sibs, indicating a high level of polymorphism and heterozygosity at RAPD loci between the two parents. Those markers that were polymorphic between parents and segregating 1:1 in the sibs, following a pseudo-testcross configuration, could be potentially useful for genetic linkage map construction of the representative species, which would lay a theoretical basis for genetic linkage map construction using RAPD markers and F_(1) population.

Key concepts: RAPD, Biology, Genetics, Loss of heterozygosity, Genetic linkage, Population, Genetic linkage map, Genetic marker

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Segregation of RAPD markers: A case in interspecific cross of Eucalyptus — Research Paper | ScholarLens