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Genetic analysis of Lycopersicon by RAPD markers

Lingxia Zhao, LI Jingfu, Kexuan Tang, Xiangyang Xu, Guoyin Kai

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Abstract

In this paper, 43 materials from 9 species in Lycopersicon were analysed by RAPD (random amplification polymorphism DNA) with 26 random primers (10bp). In total, 199 loci (stable band) were detected and averagely 7.65 loci could be detected by each random primer. The results showed that 146 polymorphic loci occupied 73.37% of the whole amplified loci. According to these results, 43 materials can be classified into 4 types and the average genetic distances of detected materials are between 0.041 and 0.452.

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What this paper is about

In this paper, 43 materials from 9 species in Lycopersicon were analysed by RAPD (random amplification polymorphism DNA) with 26 random primers (10bp). In total, 199 loci (stable band) were detected and averagely 7.65 loci could be detected by each random primer. The results showed that 146 polymorphic loci occupied 73.37% of the whole amplified loci. According to these results, 43 materials can be classified into 4 types and the average genetic distances of detected materials are between 0.041 and 0.452.

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Available abstract

In this paper, 43 materials from 9 species in Lycopersicon were analysed by RAPD (random amplification polymorphism DNA) with 26 random primers (10bp). In total, 199 loci (stable band) were detected and averagely 7.65 loci could be detected by each random primer. The results showed that 146 polymorphic loci occupied 73.37% of the whole amplified loci. According to these results, 43 materials can be classified into 4 types and the average genetic distances of detected materials are between 0.041 and 0.452.

Key concepts: RAPD, Primer (cosmetics), Biology, Genetics, Lycopersicon, Polymorphism (computer science), Genetic marker, Genotype

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