2007•Chieh P'ou Hsueh PaoRequires access
Effects of simvastatin on osteoblast VEGF expression and mice calvaria angionenesis
Yang Yun, Zhang Li-ping, LI Xiao-guang, Jianli Hu, Zuolun Zhang, Kaining Zhang, Zhang Hu, Song Shuxiang
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Abstract
Objective To explore the mechanism of osteogenesis from statins through observing the effects of simvastatin on the VEGF expression of calvaria osteoblasts and its local influence on the mice calvaria.Methods Rat calvaria osteoblasts were harvested and cultured.Cells were treated with simvastatin for 24 hours.RT-PCR and immunocytology were performed to detect the expression of VEGF.Simvastatin was injected subcutaneously over the calvaria of mice for 4 days,the calvaria were pathologically examined. Results VEGF expression in rat calvaria osteoblasts was promoted by simvastain at both transcriptional and translational levels.In vitro the calvaria thickness and angiogenesis were increased by local administration of simvastatin.Conclusion Simvastatin can promote osteoblasts VEGF expression in vitro and mice calvaria angiogenesis and in vivo,which plays an important role in stimulating osteogenesis.