The determination of total flavonoids in Shenzhujing Tablet

Objective To establish the method of determination for total flavonoids in Shenzhujing Tablet. Methods The ultrasonic extraction was used, and rutin was used as reference substance, colorimetry was used to determine the total flavonoids in Shenzhujing Tablet from different batches, by flavonoids and sodium nitrite-aluminum nitrate-sodium hydroxidc producing color under alkaline conditions. Results Rutin showed good linear relationship in the range of0.0102-0.0714 mg/m L(r = 0.9997), the average recovery was 98.80%, RSD was 0.86%(n = 9). Conclusion The method is simple, reproducible, the results are accurately and reliable and can be used to quality control of Shenzhujing Tablets.