2011•Journal of Southeast UniversityRequires access
Effects of serum of rats with LPS-induced acute lung injury on endothelial cell permeability and the protective effects of ulinastatin
Zhiyong Liu
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Abstract
Objective: To investigate the effects of serum of rats with LPS-induced acute lung injury on endothelial cell permeability and the protective effects of ulinastatin.Methods: Fifteen healthy male SD rats were divided into 3 groups,namely group A as the control group,group B with LPS-induced acute lung injury,group C with LPS-induced acute lung injury plus ulinastatin treatment.Rat pulmonary microvascular endothelial cell(RPMVEC) was isolated and culture from SD rat in vitro.The serum was collected from rats and added into RPMVEC.The effects of serum on monolayer permeability of RPMVEC were observed in transwell,and F-actin expression was evaluated by flow cytometry.Results: Serum of group B induced the increased permeability of RPMVEC monolayer and depolymerization of F-actin.Pretreatment with ulinastatin could lessen these changes.The percentage in change of permeability coefficient(△Pa%) after stimulation with the serum of rats in group A,B and C was(7.31±0.27)%,(30.13±1.24)%,(18.94±0.59)%,respectively,showing statistically significant differences(P0.05).And the fluorescent numbers of F-actin were 1 344.26±12.78,654.63±48.28,321.32±32.14,showing statistically significant differences(P0.05).Conclusion: The pro-inflammatory mediators in the serum of rats with LPS-induced acute lung injury increases RPMVEC permeability,and pretreatment with ulinastatin can lessen the hyperpermeability by inhibiting multiple pro-inflamatory mediators.