Effect of Ulinastatin injection on the permeability of pulmonary microvascular and expression of vascular endothelial growth factor in lung tissue of sepsis in rats

Objective To observe the permeability of pulmonary microvascular and expression of vascular endothelial growth factor in septic rats treated with Ulinastatin injection, and to investigate the action mechanism of Ulinastatin in pulmonary function protect.Methods A total of 30 healthy male SD rats were randomly divided into three groups, and each group had 10 rats,Group A received sham operation and the others underwent cecal ligation and puncture (CLP) operation.All the rats were accepted intraperitoneal injection of normal saline ( group A,B) or Ulinastatin (100 ku/kg).After four hours, Lung tissue express of VEGF in all rats were detected by reverse transcription-polymerase chain reaction(RT-PCR) and Enzyme-linked immunosorbent assay(ELISA) methods respectively and the permeability of pulmonary vascular were observed by injecting Evans Blue into jugular vein of rats.Results The permeability of pulmonary microvascular and the expression levels of VEGF in group B were significantly higher than those in group A and C,which in group C were significantly higher than that in group A.Conclusions Ulinastatin is a reliable protection agent for septic induce acute lung injury, and its mechanism of action may be involved in the inhibition of VEGF expression.