Analysis on Pathogenic Detection Results of 197 Clinically Diagnosed Cases with Hand-foot-mouth Disease in Hunan Province

Objective To understand type and distribution of the pathogen of hand-foot-mouth disease(HFMD),and to provide experimental basis for disease control and prevention.Methods Herpes fluid,throat swab,anal swab,stool and other specimens of clinically diagnosed cases with HFMD were collected.Virus was isolated by RD and Hep-2 cells from stool samples.Nucleic acid of three types of virus:enter virus(EV),enter virus 71(EV71)and Coxasckievirus A16(CoxA16)was detected by RT-PCR method both in orignal samples and isolated positive virus strains.Results Samples from 197 clinically diagnosed cases with HFMD were collected.105 cases were tested positive,including 72 cases of EV71 positive,14 cases of CoxA16 positive,19 cases of EV positive and EV71,CoxA16 negative.And 10 enter virus strains were isolated from stool samples including 7 strains of EV71 and 3 strains of CoxA16.Conclusion The HFMD in Hunan Province mainly caused by EV71.It accounted for 68.57% of the number of positive cases.All cities(prefectures)of Hunan Province had HFMD caused by EV71 except for Zhuzhou City and Xiangxi Autonomous Prefecture.