Determination of Polyphenols in Pomegranate Peel Extracts by Folin-Ciocalteu Colorimetry

The optimal conditions of the determination of the contents of polyphenols in pomegranate peel extracts by Folin-Ciocalteu colorimetry were investigated,and the stability,precision,repeatability and recovery of the method were tested.The optimal conditions of the determination of polyphenols in pomegranate peel extracts were obtained as follows:Folin-Ciocalteu reagent of 1.00mL,10.0g/100mL Na2CO3 of 3.00mL,the reaction temperature of 25℃,reaction time of 120min and detection wavelength of 672nm.There was a good linear relation ship between the concentration of gallic acid and absorbance in the range of 10—100μg/mL(y=0.0098x+0.0148,R2=0.9982).The method is simple,rapid and accurate,that is the reliable method for detection of total polyphenols in pomegranate peel extracts.