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Determination of Total Polyphenol in the Different Places of Pomegranate Peel

Tan Wei

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Abstract

Objective To determine the total polyphenolie in pomegrana peel by Folin-Ciocaheu colorimetry. Methods Total polyphenolie compounds in the different places of pomegrana peel were determined by Folin-Ciocaheu colorimetry(λ=760nm),and the standard reference was gallic acid. Results The linear range of standard curve was 0.0245～0.2450 mg/ml(r=0.9991,n=6),the average recovery of gallic acid was 103.3% with corresponding RSD of 1.08%,the precision RSD was 0.31%,the repetition RSD was 0.51%,and the stability RSD of 0.65%. Conclusion The method is convenient,fast,and reliable for the determination of the total polyphenolie in pomegranate peel.

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What this paper is about

Objective To determine the total polyphenolie in pomegrana peel by Folin-Ciocaheu colorimetry. Methods Total polyphenolie compounds in the different places of pomegrana peel were determined by Folin-Ciocaheu colorimetry(λ=760nm),and the standard reference was gallic acid. Results The linear range of standard curve was 0.0245～0.2450 mg/ml(r=0.9991,n=6),the average recovery of gallic acid was 103.3% with corresponding RSD of 1.08%,the precision RSD was 0.31%,the repetition RSD was 0.51%,and the stability RSD of 0.65%. Conclusion The method is convenient,fast,and reliable for the determination of the total polyphenolie in pomegranate peel.

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Available abstract

Objective To determine the total polyphenolie in pomegrana peel by Folin-Ciocaheu colorimetry. Methods Total polyphenolie compounds in the different places of pomegrana peel were determined by Folin-Ciocaheu colorimetry(λ=760nm),and the standard reference was gallic acid. Results The linear range of standard curve was 0.0245～0.2450 mg/ml(r=0.9991,n=6),the average recovery of gallic acid was 103.3% with corresponding RSD of 1.08%,the precision RSD was 0.31%,the repetition RSD was 0.51%,and the stability RSD of 0.65%. Conclusion The method is convenient,fast,and reliable for the determination of the total polyphenolie in pomegranate peel.

Key concepts: Gallic acid, Colorimetry, Chemistry, Chromatography, Polyphenol, Standard curve, Organic chemistry, Antioxidant

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