The Relevance Between Connective Tissue Growth Factor Hepatocyte Growth Factor and Vascular Remodeling of Renal Hypertension Rats

Objective: To investigate the effects of the connective tissue growth factor(CTGF)and hepatocyte growth factor(HGF) on vascular remodeling of two-kidney,one-clipped hypertensive rats.Methods: The renal hypertensive rat model was established by 2K1C using 8 weeks Wistar male rats.They were devided into two subgroup randomly with 15 each group,including control group,model group.Systolic blood pressure(SBP) of rats was detected once a week.The morphologic change were observed by HE and the ratio of common carotid artery media thickness and diameter (MT/LD)and medial membrane smooth muscle cell layers of the common carotid artery were calculated.Then the expression of CTGF and HGF were detected by immunohistochemistry.Results:①Compared with the control group,the SBP was significantly increased in the model group(P0.01).②The ratio of media thickness/diameter(MT/LD) and medial membrane smooth muscle cell layers of the common carotid artery were obviously increased in the model group(P 0.01).③The CTGF expression was higher and HGF was lower in common carotid artery in model group(P0.01).④CTGF showed positive correlation with SBP、MT/LD and media membrance cell layers(P0.01),whereas showed negative correlation with HGF(P0.01);HGF showed negative correlation with SBP、MT/LD、media membrance cell layers and CTGF(P0.01).Conclusions:In the 2K1C renal hypertensive rats model group,the CTGF participates in the pathological process of vascular remodeling in hypertension,and it is the related cytokines of vascular remodeling;the HGF inhibits the pathological process of vascular remodeling in hypertension.