Studies on Cinnamic Acid Inducing the Reversion of Malignant Phenotypes and Differentiation of Human Lung Adenocarcinoma Cells

Objective To study the differentiation effect and possible mechanism of cinnamic acid (CINN) on human lung adenocarcinoma A549 cells.Methods A549 cells were treated by CINN (2 mmol/L),The cell growth rate was determined by cell counting,and its viability was determined by trypan blue exclusion assay.And the flow cytometry (FCM) was used to analyze the cell cycle and to detect p53 protein.All experiments were done in triplicate.Results After A549 cells were incubated with CINN,the cell proliferation was markedly inhibited;The cloning efficiency on soft agar of the CINN groups was less than that of the control group;CINN may inhibit the synthesis of DNS and mitosis of A549 cells,and make cell growing in the G1/G0 phase stasis;The CINN group had less mp53 protein than the control group and the difference between them was significant ( P 0 05).Conclusions CINN could significantly inhibit the proliferatioin of A549 cells,and has certain effect on the induction of differentiation which suggest that CINN may have potential use as a DA in lung adenocacinoma intervention.The decrease of mp53 protein after CINN treatment suggest that mp53 involves in the process of induction of differentiation on A549 by CINN.