Effect of PP_(333) and CCC on growth multiplications and rooting of Toona sinensis in tissue culture

Added PP 333 or CCC at different concentrations to multiplying and rooting media when the shoots of Toona sinensis cultured.The basic medium for multiplication is MS+6-BA 0.2 mg·L -1+GA 1.0 mg.L -1,the basic media for rooting is 1/2 MS+IBA 1.0 mg·L -1.The results showed that PP 333(10 mg·L -1) or CCC(70 mg·L -1) improved the multiplication of shoots obviously,retarded the growth,the efficacy of PP 333(10 mg·L -1) is better.Meanwhile PP 333(0.1~0.3 mg·L -1) or CCC(10~70 mg·L -1) induced the callus and vitrification;PP 333 (0.1 mg·L -1) or CCC(10 mg·L -1) improved rooting rate and transplanting of the tube-plantlet,contrasted two kinds of hormons,CCC(10 mg·L -1) was evidently.After transplanting,the plant was short and strong,the colour of the leaves deepened,the rate of survitors was increased.