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[Determination of molecular weight and content of Ginkgo biloba exocarp polysaccharides by HPLC].

Ping Lü, Li Ren, Aihua Xu, Huasheng Chen

Abstract

OBJECTIVE: To determine the molecular weight and content of Ginkgo biloba exocarp polysaccharides. METHOD: The analysis was carried on a PL aquagel-OH MIXED (7.5 mm x 300 mm, 8 microm) chromatography column eluted with water as mobile phase at 1.0 mL x min(-1) of flow rate, the column temperature was 25 degrees C and the eluate was detected by RID. RESULT: The average molecular weight of Ginkgo bilobaexocarp polysaccharides was 11 062.5 with RSD = 0.78% (n = 6); the content was 81.9% with RSD = 2.5% (n = 6), the standard curves of dextran (MW 12 000) were linear in the range of 1-20 microg, r = 0.999 9. The average recovery is 97.9%, RSD was 2.5%. CONCLUSION: This method was found to be sensitive and accurate for the measurement of Ginkgo biloba exocarp polysaccharides.

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Available abstract

Key concepts: Ginkgo biloba, Polysaccharide, Chromatography, Chemistry, High-performance liquid chromatography, Ginkgo, Elution, Content determination