Protective Effect of Chlorogenic Acid on Liver Fibrosis in Rats

Objective:To study the protective effect of chlorogenic acid on liver fibrosis in rats.Method:The rats were randomly divided into 6 groups:the normal control,the model group,the colchicina group(0.2 mg·kg-1),and the chlorogenic acid groups(140,70,35 mg·kg-1).Rats in the groups were treated daily for 8 weeks.Model of liver fibrosis induced by carbon tetrachlotide(CCl4) in rats was used.To establish the model,except the rats in the normal control group,the other mice were sc injected with CCl4(5 mL·kg-1) as the first attack and then sc injection of peanut oil of 40% CCl4(3 mL·kg-1) twice a week in the following 8 weeks.The indexes of live and spleen in rats were observed.After blood sample collection,alanine aminotransferase(ALT) and aspartate aminotransferase(AST) activities and content of total protein(TP),albumin(ALB) in serum were examined,meanwhile the ratio of the ALB and globulin(GLOB) was calculated.The four indicators of liver fibrosis hyaluronic acid(HA),laminin(LN),procoliagen type Ⅲ(PCⅢ),collagen type Ⅳ(CⅣ) were determined in liver tissue.Superoxide dismalase(SOD) activity and malondial-dehyde(MDA),glutalhioneperoxidase(GSH-Px) levels in liver tissue were determined.Result:Chlorogenic acid could obviously decrease the index increase of the live and spleen(P0.01) in liver fibrosis of rat,remarkably resist the increase of ALT and AST in serum(P0.01),increase the content of TP,ALB,A/G in serum(P0.01),suppress the content of HA,LN,PCⅢ,CⅣ,GLOB in liver tissue(P0.01),decrease the MDA levels in liver tissue(P0.01),and improve SOD activity and GSH-Px level in liver tissue(P0.01).Conclusion:Chlorogenic acid has significant protective effect on liver fibrosis in rats.