Process Optimization for Enzymatic Hydrolysis of Hemoglobin in Sika Deer Blood

The anticoagulant venous sika deer blood was hydrolyzed by different enzymes and hydrolysis conditions to examine hydrolysis degree,nitrogen recovery and content of hemin in hemoglobin hydrolysate.Step hydrolysis process with Alcalase and Flavorzyme was optimized to get good quality and hemin enriched hydrolysis product.Enzyme type and enzymatic hydrolysis mode had obvious influence on hydrolysis degree and nitrogen recovery.Step hydrolysis with Alcalase and Flavorzyme obtained a significant effect on sika deer blood.The suitable hydrolysis temperatures of Alcalase and Flavorzyme were 55 ℃ and 50 ℃,and initial pH values were 8.0 and 6.5,respectively.For Alcalase and Flavorzyme,the suitable substrate concentration and proteases dosage were 8% and 6 000 U/g,respectively.When 8% substrate(pH 8.0) was hydrolyzed by 4 500 U/g of Alcalase for 1h and followed by 1500 U/g of Flavorzyme for 3 h,the hydrolysis degree,nitrogen recovery and hemin content of hydrolysate were 27.95%,73.75% and 3.326 mg/mL,respectively.