The calibration and comparative test of BC-5500 automated hematology analyzer

Objective TO calibrate and compare the new arrival BC-5500 automated hematology analyzer using fresh whole blood,insure the results of specimen can trace to the resourse of same measure basis.Methods The fresh whole blood of healthy volunteer had done physical examination was collected and detected with F-820 and BC-5500 hematology analyzer,the F-820 hematology analyzer was as target system;counted the deviations of various parameters about BC-5500 hematology analyzer.According to the standard for allowable error,the decision was made if the calibration to BC-5500 hematology analyzer was needed.When all the parameters were conformed to the standard,we compared the hematology analyzers with fresh whole blood of clinical parents.Results Before the calibration,all the parameters were outside the ranges,white blood-cell count(WBC)、red blood-cell count(RBC)、haemoglobin(HB)、mean corpuscular volume(MCV)、platelet(PLT):11.2%、3.4%、2.5%、1.97%、13.5%;after the calibration,all the parameters were conformed to the standard,WBC、RBC、HB、MCV、PLT:0.02%、0.4%、0.62%、0.43%、0.84%.In the comparative test,the coincident rate of RBC、HB and MCV was 80%,then WBC and PLT was 90%.THE calibration factors can be acceptable.Conclusion The method that useing fresh whole blood calibrate hematology analyzer is efficiently,but the comparative test must be done to insure the parameters conformed to the accreditation of clinical Laboratory.