ScholarLens Back to search
2006•Parenteral & Enteral NutritionRequires access

The effects of glutamine on the apoptosis of pancreatic acinus in rats with SAP

Zhaohan Wu

Open publisher page 0 citations

Abstract

Objective:To investigate the role of glutamine on apoptosis of pancreatic acinus in SAP rats.Methods:Eighty SD rats were randomly allocated to 3 groups :glutamine group(Gln group,n=36),SAP group(n=36) and control group(n=8).Jejunotomy was performed.Gln group received glutamine,and SAP group received saline.All groups were subdivided into 3 groups.Eight normal rats in control group were used as controls.At 12,24 and 36 hours after the induction of SAP,twelve rats in each group were sacrificed.Plasma and pancreatic acinar tussue glutamine concentrations were measured by HPLC.The apoptosis of pancreatic acinar cells was detected by TUNEL method.Results:The apoptotic rate of pancreatic acinar in SAP group was much higher than that in control group(P0.01),and it was higher in Gln group than that in SAP group(P0.01).Conclusion:Enteral administration of glutamine can increase the apoptotic rate of pancreatic acinar cells in SAP rats.

About this research paper

What this paper is about

Objective:To investigate the role of glutamine on apoptosis of pancreatic acinus in SAP rats.Methods:Eighty SD rats were randomly allocated to 3 groups :glutamine group(Gln group,n=36),SAP group(n=36) and control group(n=8).Jejunotomy was performed.Gln group received glutamine,and SAP group received saline.All groups were subdivided into 3 groups.Eight normal rats in control group were used as controls.At 12,24 and 36 hours after the induction of SAP,twelve rats in each group were sacrificed.Plasma and pancreatic acinar tussue glutamine concentrations were measured by HPLC.The apoptosis of pancreatic acinar cells was detected by TUNEL method.Results:The apoptotic rate of pancreatic acinar in SAP group was much higher than that in control group(P0.01),and it was higher in Gln group than that in SAP group(P0.01).Conclusion:Enteral administration of glutamine can increase the apoptotic rate of pancreatic acinar cells in SAP rats.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To investigate the role of glutamine on apoptosis of pancreatic acinus in SAP rats.Methods:Eighty SD rats were randomly allocated to 3 groups :glutamine group(Gln group,n=36),SAP group(n=36) and control group(n=8).Jejunotomy was performed.Gln group received glutamine,and SAP group received saline.All groups were subdivided into 3 groups.Eight normal rats in control group were used as controls.At 12,24 and 36 hours after the induction of SAP,twelve rats in each group were sacrificed.Plasma and pancreatic acinar tussue glutamine concentrations were measured by HPLC.The apoptosis of pancreatic acinar cells was detected by TUNEL method.Results:The apoptotic rate of pancreatic acinar in SAP group was much higher than that in control group(P0.01),and it was higher in Gln group than that in SAP group(P0.01).Conclusion:Enteral administration of glutamine can increase the apoptotic rate of pancreatic acinar cells in SAP rats.

Key concepts: Glutamine, Acinus, TUNEL assay, Apoptosis, Medicine, Acinar cell, Internal medicine, Saline

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
The effects of glutamine on the apoptosis of pancreatic acinus in rats with SAP — Research Paper | ScholarLens