Application Value of Detection of High Risk Human Papillomavirus DNA in Screening for Cervical Precancerous Lesions

[Objective]To evaluate the application value of high risk human papillomavirus(HPV) DNA in screening for cervical cancer.[Methods] During 2005-2007,a total of 1240 married women who conducted health examination in Lianyungang First People's Hospital(1026 cases of non-clinical symptoms,214 cases of gynecologic symptoms) and 534 cases of cervical abnormalities were screened cervical precancerous lesions by high risk HPV DNA test and liquid based Thinprep cytology test(LTCT);Youden index was adopted to conduct comparison between screening results of HPV·DNA and LTCT based on the histopathological diagnosis as the gold standard.[Results] Of 1774 patients,the average age was(35±10.2) years,51(3.24%) were pathologically diagnosed HPV,49 were cervical intraepithelial neoplasia(CIN)level I,26 were level II,17 were level III,6 were invasive cervical cancer,2 were endometrial cancer,26 were condyloma.Based on histopathological diagnosis as gold standard,the sensitivity,specificity,positive predictive value,negative predictive value and Youden index of HR HPV·DNA test for detecting CIN Ⅱ,Ⅲ were 95.35%,91.67%,22.16%,99.87%,and 0.87 respectively;that of HR HPV·DNA test for detecting +LTCT were 100.0%,100.0%,100%,and 1.0 respectively.The positive rates of HPV in healthy women and patients with cervical lesions were normal(inflammation) 4.80%,ASCUS 50.67%(38/75),LSIL 94.44%(34/36),HSIL 100%(31/31),invasive carcinoma 100%.[Conclusion] HR HPV·DNA test has high sensitivity and negative predictive value in detection of cervical precancerous lesions and cervical cancer screening.The combination of LTCT and HR-HPV·DNA test increases the sensitivity and negative predictive value of screening.