Effect of bone marrow stem cells transplantation on heart function of acute myocardial infarction rats

Objective To investigate the effect of bone marrow stem cells(MSCs) transplantation at different concentrations on heart function of acute myocardial infarction(AMI) rats.Methods AMI was created by occluding the left anterior descending artery(LAD) in SD rats.MSCs of healthy rats were isolated,purified,amplified and labeled with 4',6-diamidino-2-phenylindole(DAPI).Forty-eight rats were randomized to six groups.Two weeks after MI,the labeled MSCs were transfused to AMI rats through caudal vein at dosage of 5×105(A),1×106(B),5×106(C) and 1×107(D),5×106 MSCs were injected via epicardium for group E and culture medium of the same volume was transfused to control group.By the end of the fourth week,the heart functions were evaluated and immunohistochemical analysis was performed.Results The labeled cells were viable in the host hearts and immunochemical staining revealed that the engrafted stem cells expressed α-actin.There was significant difference in left ventricular function and infarction area in groups C and E compared with control group the infarction areas of groups C,E and control group were[(41±3)%,(40±4)% and(45±2)%,P0.05].Compared with that of control group,the left ventriclar function had no difference in group A and was more obviously improved in groups B,C,D and E(P0.05).Among the transplantation groups,the groups C and D had greater improvement than group B(P0.05).Conclusions MSCs can migrate into the infarcted areas through different ways and improve the heart function.