Comparison study of hepatic fibrosis in rats induced by carbon tetochloride and tioacetamide

AIM:To induce hepatic fibrosis in rats by CCL4 and TAA respectively to compare the advantages and disadvantages of both methods.METHODS:Sixty male Sprague-Dawly rats were randomly divided into four groups.Hepatic fibrosis was induced in the ways introduced in references.Control groups were set respectively.The animals were sacrificed on day 28 and 56 after weighed.Serum was collected to examine the level of ALT,AST,HA and CIV and livers were homogenized to examine the level of SOD and MDA.The liver inflammation and collagen deposition were observed with HE and Masson's collagen stains and analyzed with scoring and staging systems.RESULTS:On day 28,significant differences in observed indexes were found between control and model groups,but no difference was seen between the 2 model groups.On day 56,significant differences was observed between the two model groups.CONCLUSION:The animal model of hepatic fibrosis induced by CCL4 is more suitable for researches on the mechanism of spontaneous regression of hepatic fibrosis and related strategies for treatment.The animal model of hepatic fibrosis induced by TAA is more suitable for researches on the mechanism of liver fibrosis,evaluation of antifibrotic effects of medicine,and evaluation of reliability of serous markers for the diagnosis of liver fibrosis.