Analysis of the distribution and drug resistance of pathogenic bacteria in blood cultures

Objective To investigate the distribution of pathogenic bacteria in the blood culture,and observe the drug resistance of common pathogenic bacteria in our hospital for empirical antimicrobial therapy.Methods The blood cultures Bact/Alert120 system was applied for culture.The species identification and antibiotic resistance tests were performed with the VITEK-32 automicroscan system.Results Among the 10 291 clinical samples,totally 651 strains of pathogenic bacteria were isolated.49.62%(n=323) were gram-positive cocci,40.70%(n=265) were gram-negative cocci,and 9.68%(n=63) were fungi.The coagulase negative staphylococcus isolates accounted for 63.78% of all gram-positive isolates and 31.64% of all isolates.The second was enterococcus isolates accounted for 19.10% of all gram-positive isolates and 7.99% of all isolates.E.coli was dominant,accounted for 18.13% of all isolates and 44.15% of all gram-negative isolates.About 83.98% of S.aureus and 59.26% of coagulse negative staphy-lococcus were methicillin-resistant.No vancomycin-resistant was found.Staphylococcus was resistant against penicillin and ampicillin,which was higher than gentamycin.The prevalence of extended-spectrum β-lactamases was 56.78% in E.coli,40.00% in K.pneumoniae.The resistance rate of staphylococcus was lower than imipenem and cefoxitin.P.aeruginosa was susceptible to imipenem,ceftazidime,gentamycin and netilmicin.The drug resistance of it to other kinds of antibiotics were higher.Conclusion The most pathogenic bacteria isolated from blood specimens are opportunistic antibiotics pathogens,and drug resistance of isolated pathogenic bacteria is a serious problem.Monitoring the change of pathogen and the trends of antimicrobial resistance is very important in guiding the clinical administration.