Investigation of species specificity on 15 PCR-based human STR systems

Objective To evaluate species specificity of 15 STRs loci of 9 different kinds of animials. Methods Human DNA and 15 different kinds of common animals' DNA were amplified separately by PCR.The PCR products were analyzed with ABI310. Results There were amplified products in D8S1179,CSF1PO,D19S433 loci of the nine kinds of animals.The lengths of amplified fragment were distinctly different between human and animals in TPOX loci.There were amplified products in some kinds of animals in D21S11,D7S820,D13S317,D16S539,TPOX,D18S51,D5S818,FGA,D3S1358 loci.There were not amplified products in D2S1338,vWA,TH01 loci of these nine kinds of animals. Conclusion In 15 STR loci,3 has good species specificity,which can be amplified in human;9 can be amplified in human and partial animals,but the amplified fragment length are different between human and animals,and can be used to analyze DNA species in individual identification;3 can be amplified in human and animal,but the fragment lengths are familar,and the DNA species should be determined first in individual identification.