The Clinical and pathological significance of expression of VEGF and CD34 in gastric carcinoma and MVD

Objective The current study was designed to investigate the expression of VEGF and CD34 in gastric carcinoma and clinical and pathological significance of MVD.Methods The expression of VEGF in surgical samples of 45 patients with gastric carcinoma and 20 patients wth superficial gastritis were determined by SP immunohistochemical staining method. Intratumoral VEGF and MVD were stained with anti-VEGF and vascular endothelial cells were displayed by anti-CD34 monoclonal antibody and positive vessels of them were made a MVD count. Results The positive expression of VEGF was stained mainly in the cytoplasm of cells. VEGF-positive expression percentage and the mean MVD in gastric carcinoma tissue was significantly higher than that superficial gastritis. There is significant correlation between the expression of the MVD and TNM stage,the invasive depth,lymph node metastasis( P 0.05).No significant correlation was found between the expression of the MVD and degree of differentiation( P 0.05).The MVD in VEGF-positive patients was significantly higher than that in VEGF-negative patients( P 0.05).Conclusion There are high expression of VEGF and CD34 in gastric carcinoma,the former may promoted the progression of lymph node metastasis and angiogenesis of gasric carcinoma.