2012•Tobacco Science & TechnologyRequires access
Serological Identification of Viruses Infecting Tobacco in Major Tobacco Growing Areas of China
Zhaopeng Luo, Jun Yang, Ran Wang, Feng Li, Lifeng Jin, Wei Chunyang, Lin Fucheng
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Abstract
In order to better understand the occurrence of tobacco virus diseases in China status quo,409 symptomatic tobacco samples were collected from 13 major tobacco growing areas of China in 2010 and 2011.Tobacco mosaic virus(TMV),cucumber mosaic virus(CMV),potato virus Y(PVY) and tobacco etch virus(TEV) were detected by ELISA(enzyme-1inked immunosorbent assay).The results showed that the infection rate of TMV was the highest,which was 64.6% and 48.2% in 2010 and 2011,respectively;that of CMV was 32.1% and 28.2%,respectively;and that of PVY was 15.6% and 11.4%,respectively;while that of TEV was the lowest.There presented 7 types of mixed infection.The mixed infection of TMV+CMV topped the highest rate of 34.9% and 28.6% in 2010 and 2010,respectively.It indicated that TMV and CMV were the predominant viruses infecting tobacco in China,and the mixed infection occurred seriously in tobacco field.