Alcohol-dispelling Effect of BUZUIDAN and Its Underlying Mechanism

Objective To observe alcohol-dispelling effect of BUZUIDAN on drunken mice,and explore the mechanism of action.Methods: ①Male BALB/c mice were divided into three groups randomly: normal saline group,KingDrink group and BUZUIDAN group.Each mouse was administered with Beijing spirit er guo tou by gastric perfusion for three times,0.06mL once at 10 minutes intervals,and the total volume was 0.18mL.Following each alcohol administration,0.06ml of normal saline,KingDrink and BUZUIDAN were respectively fed to each mouse of the corresponding group immediately by gastric perfusion.The time period were recorded for each mouse getting drunk after the first alcohol administration,as well as the number of drunken mice.②Repeating the experiments mentioned above,effects on plasma alcohol concentration in mice.Blood were collected and plasma was dissociated from each mouse in 50 minutes following the first alcohol administration,and then alcohol concentration was determined.Results: ① The number of the drunken animals was 2/10 both in BUZUIDAN group and in KingDrink group,which was obviously less than that in the normal saline group with 9/10 drunken animals.There is no significant difference in the mean time of animal getting drunk when the BUZUIDAN group and KingDrink group were compared to the normal saline group;②Plasma alcohol concentration of the mice in the BUZUIDAN group was lower than that in the normal saline group(P 0.05)significantly.Conclusion: BUZUIDAN can effectively relieve drunkness in mouse,and the mechanism of effects was possibly associated with lowering plasma alcohol concentration.