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2010•Journal of Hefei Normal UniversityRequires access

Application of PCR-DGGE in Study of Microbial Community

Feng Weisong

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Abstract

PCR-denaturing gradient gel electrophoresis(PCR-DGGE) has the advantages of high reliability,good repetition,and easy operation.Currently,it has been widely used in the study of diversity and dynamics of microbial communities.This paper introduces the principle and procedure of PCR-DGGE,discusses the optimization and statistical analysis of PCR-DGGE profile,reviews its application in the study of environmental microbial ecology,and analyzes its limitations and potential application.

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What this paper is about

PCR-denaturing gradient gel electrophoresis(PCR-DGGE) has the advantages of high reliability,good repetition,and easy operation.Currently,it has been widely used in the study of diversity and dynamics of microbial communities.This paper introduces the principle and procedure of PCR-DGGE,discusses the optimization and statistical analysis of PCR-DGGE profile,reviews its application in the study of environmental microbial ecology,and analyzes its limitations and potential application.

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Available abstract

PCR-denaturing gradient gel electrophoresis(PCR-DGGE) has the advantages of high reliability,good repetition,and easy operation.Currently,it has been widely used in the study of diversity and dynamics of microbial communities.This paper introduces the principle and procedure of PCR-DGGE,discusses the optimization and statistical analysis of PCR-DGGE profile,reviews its application in the study of environmental microbial ecology,and analyzes its limitations and potential application.

Key concepts: Temperature gradient gel electrophoresis, Microbial population biology, Biology, Microbial ecology, Ecology, Biochemical engineering, Computational biology, Genetics

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