Condition Optimization of PCR-DGGE in the Research of Bacterial Diversity

As a reliable,reproducible rapid and inexpensive molecular method,PCR-DGGE had been proven to be a powerful tool for studying microbial communities and their dynamics changes.This technique was widely used in environmental ecology to analyze the diversity and dynamics of microbial communities,and to monitor functional bacteria without isolation and cultivation processes.In this paper,the principle,as well as the limitations and future perspectives of DGGE techniques for microbial ecology analysis were introduced,and the main causing factors addressed and discussed,such as DNA extraction,PCR amplification,DGGE conditions optimization.