Rapid detection and molecular characterization of type 1 Streptococcus suis of first human infection case in Jiangsu Province

Objective To apply rapid detection and molecular characteristics of pathogen in 1suspected-Streptococcus suis infection case in Jiangsu Province,2014.Methods DNA was extracted from patient's specimens.PCR was used to detect specific gene of Streptococcus suis serotype 2and identification of virulence genes followed by multi-locus sequence typing analysis.Results Streptococcus suis specific gene(16srRNA)and serotype 2specific gene encoding Capsular polysaccharide(cps-2J),together with 5virulence genes(mrp,sly ef,gapdh and fbps)were all positive in PCR reactions.Multi-locus sequence typing analysis showed pathogen was type 1 Streptococcus suis(ST1).Conculsion It was a case of human infection with type 1Streptococcus suis of serotype 2carrying 5virulence genes,which was first reported in Jiangsu Province.