Inhibition effect of genistein on proliferation of prostate carcinoma PC-3 cells

Objective To study the influence of Genistein on the growth of prostate carcinoma PC-3 cells in vitro.Methods Prostate cancer cells were grown in DMEF 12 medium supplemented with 10% bovine serum and 5?ml 10?000?U/ml of penicillin/streptomycin in an incubator maintained at 5% CO_2 and 37℃.Using cell culture tests(MTT test,index of cellular mitosis,Cloning formation and()~3H-TdR incorporation test),We examined the growth of PC-3 cells at four concentrations genistein 10,20,40,80?μmol/L with a negative control(anhydrous alcohol and DMSO v/v).Results Genistein could inhibied the growth of PC-3 cells.After treatment with various geinistein concentrations mnentioned above for 7 days,the inhibition rates were 27.7%,58.8%,72.3% and 84.4% respectively.With the increase of genistein concentrations,Mitotic index and Cloning formations were decreased in PC-3 cells and the quantity of()~3H-TdR incorporation was significantly decreased as compared with the negative control(P0.05).Conclusion Genistein can significantly inhibit the growth of PC-3 cells and it is probably one of the preventive mechanisms of genistein in prostate cancer.