Determination of the AmpC and ESBL produced in the biofilm of klebsiella pneumoniae

AIM:To evaluate the drug-fast mechanism of bacteria growing in biofilm,and to provide the theoretical basis for selecting antimicrobial agents in clinic.METHODS:The model of klebsiella pneumoniae bacterial biofilm were built up with the modified flat-board method and identified with the method staining with AgNO3 and confocal scanning laser microscopy.The ESBLs and AmpC were detected by improved three dimensional test.RESULTS:The detection rates of AmpC,ESBLs and AmpC plus ESBLs in isolated klebsiella pneumoniae were 2.5%(1/40),20.0%(8/40)and 2.5%(1/40),whereas the detection rates of AmpC,ESBLs and AmpC plus ESBLs in biofilm klebsiella pneumoniae were 20.0%(8/40),45.0%(18/40)and 22.5%(9/40).The resistance rates of the biofilm klebsiella pneumoniae producting AmpC and ESBLs to eight kinds of antibiotics were higher.CONCLUSION:The synergetic effect of the formation of biofilm and the production of ESBLs and AmpC is one of the main reasons that klebsiella pneumoniae resists antibiotics.