Effect of fluoride and calcium on serum LPO in ovariectomized rats

Objective To evaluate the effect of fluoride and fluoride plus calcium on serum LPO in ovariectomized rats. Methods Sixty-four female Wistar rats of three months were divided randomly into sham-operated group (sham), ovary resection group (OVX), after ovary resection given fluoride group (OVXF), and after ovary resection given fluoride plus calcium group (OVXFCa).Fluoride-added water was given in OVXF group after ovary resection once every other day, the fluorine content in water was 2mg、4mg、6mg per liter. OVXFCa group received the same fluoride as OVXF group, simultaneously with calcium gluconate, in each group, calcium ion content in water was 900mg per liter. After six months feeding, all animals were killed, and serum LPO was tested by TBA assay.Results There was a significant increase in serum LPO (nmol/L) in OVX group as compared with the sham group ( P 0.05 ). The content of serum LPO in OVXF and OVXFCa group was significantly lower than that in OVX group ( P 0.001),but there was no distinct difference between OVXF group and OVXFCa group in serum LPO( P 0.05).Conclusion The content of rat serum LPO in ovary resection group increased remarkably and the lipid peroxidation degree of ovariectomized rats could be improved after given different dose fluoride and different dose fluoride plus calcium. It is suggested that fluoride and fluoride plus calcium may improve the antioxide capability of human body in curing osteoporosis.