Hemostatic effects of several hemostatic agents on parenchymatous organ trauma in rabbits

Objective To evaluate the hemostatic effects of topical hemostatic agents: gelatin sponge,absorbable hemostatic gauze,composite sponge and quick-acting styptic powder(FlashclotTM) on parenchymatous organ trauma in rabbits,and histological changes induced by these hemostatic agents.Methods A parenchymal organ active hemorrhage model was established by transecting the livers and spleens of rabbits about 1.0 cm away from anterior extremity under the condition of normal and liquemine anticoagulation respectively.Various hemostatic materials were dressed on the wound surface separately,and the bleeding time and blood lose were accurately recorded.Tissue samples were transected from the livers contacted with dressings for histology analysis.Results Under normal condition,the hemostatic time of gelatin sponge,composite sponge,absorbable hemostatic gauze,and FlashclotTM was(6.28±1.45)minutes,(6.19±1.04)minutes,(5.09±1.03) minutes and(4.07±0.84) minutes respectively,obviously shorter than that of common gauze(9.89±0.75)minutes(P0.05).Under the condition of liquemine anticoagulation,the hemostatic time of all groups increased obviously,especially the groups of gelatin sponge and absorbable hemostatic gauze.The result of histology analysis showed that FlashclotTM resulted in heat injury to the liver trauma area.Conclusion Both composite sponge and FlashclotTM present better hemostatic efficiency than gelatin sponge and absorbable hemostatic gauze,especially under the condition of liquemine anticoagulation.But FlashclotTM should only be applied to emergency hemostasis of life-threatening trauma due to the heat injury and difficulty in removing.