2009•ZhongcaoyaoRequires access

HPLC Fingerprints of volatile oil of Curcuma wenyujin in Zhejiang Province

Xiaokun Li

Abstract

Objective To establish the chromatographic fingerprint of Curcuma wenyujin by HPLC.Methods The chromatographic fingerprint of C.wenyujin was established by HPLC with Shimadzu VP-ODS C18 column(250 mm×4.6 mm,5 μm),acetonitrile-water(0.1 phosphoric acid) gradient elution,the flow rate of 1.0 mL/min,column temperatures of 30 ℃,and detective wavelength at 210 nm.ResultsTaking germacrone as the reference peak,14 common-peaks were selected as the fingerprint peaks.The similarities among samples of C.wenyujin among 19 approved samples and their standard chromatographic fingerprints were calculated with a software Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica published by GPC(Version 2004A) and cluster analysis.ConclusionThis method with better accuracy and reproducibility could be used in the quality control of C.wenyujin.

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Key concepts: Chromatography, Reproducibility, Chemistry, Fingerprint (computing), Phosphoric acid, High-performance liquid chromatography, Gradient elution, Analytical Chemistry (journal)