Research on extraction condition and anti-oxidation of jujube polysaccharide

The extraction conditions of jujube polysaccharide were optimized by designing orthogonal experiment,and the extraction rates of jujube polysaccharide were 11.33% with water soak method,12.40% with ultrasonic method and 10.98% with microwave method under the optimal conditions.The study on anti-oxidation confirmed that in the concentration range of 0.075mg/mL to 0.135mg/mL the maximum scavenging ratio was 48.5%(microwave),47.1%(water soak) and 28.2%(ultrasonic) respectively.Three kinds of jujube polysaccharide extracts could scavenge.OH in vitro,which showed the relationship to dose-dependence,but the anti-oxidation of jujube polysaccharide extracted with the different extraction method has the significant deviation.It shows that the bioactivities of jujube polysaccharide depend on the extraction condition.In view of these results,the microwave method has the best effect in the extraction of jujube polysaccharide by synthesizing extraction process,extraction rates and anti-oxidation efficiency.