Determination of nucleotides in infant formula milk powder by reversed-phase ion pair chromatography

For the determination of 5 nucleotides amount in infant formula milk powder,the Reversed-Phase Ion Pair Chromatography detector method was established.We established the method in which 10% acetic acid was used for elimination of protein,C18 column and the liquid phase of 1.4 mmol/L Tetarbutylammonium hydrogen sulfate(0.01 mol/L KH2PO4 and pH=4.0)for isolation and the nucleotides were determined quantitatively by standard curve method at the UV 254nm and the flow of 1 mL/min.The average recovery rates of cytidine,uridine,adenosine,guanosine and inosine were 95.7%、96.0%、90.8%、98.7%、99.3%,respectively and the coefficient variance were 0.583%、0.147%、0.257%、0.387%、0.167%.Without SPE,the method was able to quickly,accurate and sensitive detect nucleotides amount in infant formula milk powder.