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2009•Zhongguo rupin gongyeRequires access

HPLC method for determination of nucleotides in infant formula milk powder

Zhang QiuMei, Yanping Hao, Song Ge

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Abstract

For the determination of 5 nucleotides in infant formula milk powder,the HPLC UV absorbance detector method was established.We established the method in which 10% acetic acid was used for elimination of protein,SUPELCOSIL LC-18-T column for isolation and the nucleotides were determined quantitatively at UV254 nm wavelength with HPLC.The mobile phase was phosphate and methanol.The average recovery rates of cytidine,uridine,adenosine,guanosine and inosine were 93.2%,103.4%,106.2%,94.8%,103.7%,respectively and the coefficient variance were 1.4%,1.6%,1.7%,1.8% and1.7%.

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What this paper is about

For the determination of 5 nucleotides in infant formula milk powder,the HPLC UV absorbance detector method was established.We established the method in which 10% acetic acid was used for elimination of protein,SUPELCOSIL LC-18-T column for isolation and the nucleotides were determined quantitatively at UV254 nm wavelength with HPLC.The mobile phase was phosphate and methanol.The average recovery rates of cytidine,uridine,adenosine,guanosine and inosine were 93.2%,103.4%,106.2%,94.8%,103.7%,respectively and the coefficient variance were 1.4%,1.6%,1.7%,1.8% and1.7%.

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Available abstract

For the determination of 5 nucleotides in infant formula milk powder,the HPLC UV absorbance detector method was established.We established the method in which 10% acetic acid was used for elimination of protein,SUPELCOSIL LC-18-T column for isolation and the nucleotides were determined quantitatively at UV254 nm wavelength with HPLC.The mobile phase was phosphate and methanol.The average recovery rates of cytidine,uridine,adenosine,guanosine and inosine were 93.2%,103.4%,106.2%,94.8%,103.7%,respectively and the coefficient variance were 1.4%,1.6%,1.7%,1.8% and1.7%.

Key concepts: High-performance liquid chromatography, Chromatography, Guanosine, Chemistry, Cytidine, Inosine, Nucleotide, Uridine

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