Effect of TFMMT on the immune functions in immune-depressed mice

Objective To test immunoregulatory activity of N-[2-(5methoxy-2-ethoxycarboryl-1H-indol-3-yl)-ethyl]trifluoroacetamide(TFMMT) in the immune-depressed mice. Methods Immune depression mice models were used in the experiment,and immune depression model was established by cyclophosphamide.Phagocytosis functions of splenic macrophages,spleen NK cell activity,lymphocyte transformation,IL-2 releasing determination and the ability of antibody formation were also analyzed. Results TFMMT could oppose the decrease of the phagocytose activity of macrophage,enhance NK cell activity.The high doses of TFMMT also increased the ability of antibody formation on immune-depressed mice model induced by CY. TFMMT increased splenic lymphocyte transformation stimulated by ConA in doses between 1.17×10-9-3.49×10-6 mol·L-1,and it also promoted the secretion of IL-2 in spleen. Conclusions TFMMT can protect the immune function in immune depression mice model induced by CY than that in normal ones.