Effects of Eight Weeks Interval Exercise and G-CSF Mobilization on VEGF/VEGFR-2 of Myocardial Tissue in Rats with Myocardial Infarction

Objectives: This study explored the effects of interval exercise and granulocyte colony-stimulating factor( G-CSF) mobilization on angiogenesis density and the expression of angiogenesis pathway factor VEGF/VEGFR-2of myocardial tissue in rats with MI. Methods: Male 3-month old SD rats weighted 185- 210 g were subjects and were established MI model by ligation of the left anterior descending coronary artery( LAD). Survival rats were randomly divided into sham-operated group( group A),sedentary MI group( group B),interval exercise with MI group( group C),G-CSF with MI group( group D) and interval exercise with G-CSF plus MI group( group E),10 rats in each group. Rats in group C and group E were underwent 8-week treadmill interval exercise. After exercise,the expressions of VEGF and VEGFR-2 and number of CD31 in myocardial tissue were tested by Immunohistochemistry,and the formation of blood vessels in MI area was observed by ink perfusion method. Results: The results of Immunohistochemistry indicated that all the intervention can improve the expressions of VEGF and VEGFR-2,and number of CD31 in myocardial tissue,and they were highest in E group,following by D group,and C group was the lowest one. The results of ink perfusion method showed that all the interventions can promote vascular and vascular network formation in MI area. Conclusions: Interval exercise or G-CSF can promote the expression of vascular regeneration pathway of VEGF / VEGFR-2,accelerate angiogenesis,and combining both of them has better effect,which is related to that both of them can improve the mobilization and differentiation of endothelial progenitor cells( EPCS). This study provides experimental basis for treatment of ischemic heart disease.