In Vitro Anti-tumor Effects of Chloroform Extract from Trametes versicolor Fruiting Body

In this report,in vitro the inhibition and cytotoxic effects of a chloroform extract from Trametes versicolor fruiting body(CVFC) was studied using a liver carcinoma HepG2 cell line.MTT assay results showed the CVFC inhibited HepG2 cells growth in a dose-dependent manner.The IC50 was found as(75.83±3.96) μg·mL-1.Cell morphological observation using fluorescent microscope and transmission electron microscope revealed that the CVFC treated cells presented typical apoptotic characteristics,including cell shrinkage,nuclear condensation and fragmentation.Annexin V/PI staining assay confirmed that when HepG2 cells treated with CVFC at a concentration of 150 μg·mL-1 for 24 h,the cell membrane phosphatidylserine flipped out,which was a golden mark of apoptosis.The percentage of early and late apoptosis cells were 17.82% and 22.59% respectively,much higher than that of the control(5.35% and 3.75% respectively).These results suggested that CVFC had an inhibition effect on HepG2 in vitro and mechanism may be through inducing apoptosis.