Effects of Ricinus communis Root Extract on proliferation and Apoptosis of HepG2,NCI-H460 and SGC-7901 cell

Effects of Ricinus communis root extracts on proliferation and apoptosis in human hepatoma cell lines HepG2,lung cancer cell lines NCI-H460 and gastric cancer cell lines SGC-7901 were investigated.Cell proliferation rate of different root extracts on HepG2 cells,NCI-H460 cells and SGC-7901 cells was determined by MTT assay.The apoptosis of HepG2 cells was observed by fluorescent dye staining with Hoechst 33258.HepG2 cell cycle was measured by flow cytometry.The results showed that petroleum ether extract of R.communis root had a strong inhibitory effect on proliferation of HepG2 cells,NCI-H460 cells and SGC-7901 cells,the IC50 respectively were 88.6,134.3 and 138.1 g/mL in 48h,the IC50 were respectively 65.6,133.3 and 136.6 μg/mL in 72h.Ethyl acetate extract also had a moderate inhibitory effect on proliferation of HepG2 cells,NCI-H460 cells and SGC-7901 cells in 72 h,the IC50 respectively were 90.2,138.5 and 188.2 μg/mL.Chloroform extract had mild intensity to the proliferation of NCI-H460 cells and SGC-7901 cells in the 72 h,but chloroform extract had no inhibition to HepG2 cell proliferation.The Hoechst 33258 fluorescence dyeing demonstrated that petroleum ether extract(60 μg/mL) could make the HepG2 cell appears apoptosis.Flow cytometry analysis showed that petroleum ether extract(60 μg/mL) could arrest HepG2 cells in S phase(compared with control group,P0.05).