Changes of fasting FFAs and insulin sensitivity in obesity rats induced by high-fat diet

Objective:To observe the changes of fasting FFAs and insulin sensitivity in obesity rats induced by high-fat diet.Methods:Normal male Wistar rats(9weeks old)were divided into two groups taking either norml chow(NC,3.3Kcal/g,15～20%calories for fat)or high-fat diet(HF,5.1Kcal/g,61%calories for fat).10weeks later,body weight,visceral fat mass,fasting glucose,fasting insulin and FFAs were de-termined,and HOMA-IR was calculated by the method HOMA-IR=FPG×FINS/22.5.Results:Rats of HF showed significantly that body weight and visceral fat mass gained(body weight increased by11.4%in HF compared with that in NC,P0.05.Visceral fat mass increased by20.7%in HF compared with that in NC,P0.01)and the insulin resistance occurred(HOMA-IR:HF:8.88±4.25vs NC:3.92±2.26,P0.05)after10weeks feeding.The concertration of fasting plasma FFAs was also increased significantly in HF group compared with NC group by80.8%(P0.05).But there was no difference in fasting plasma glucose in the two groups(HF:7.89±1.46mmol/Lvs NC:8.70±1.59mmol/L,P0.05).Conclusion:In this study,obesity rat with insulin resistance was induced successfully with high-fat diet,and the state of insulin resistance of obesity rats had correlation with the increased concentration of fasting plasma FFAs.