Separation and Screening Proteins Associated with Resistant to Schistosoma japonicum Infection from Tissues and Serum of Microtus fortis

In order to comprehensive compare and screen potential proteins resistance to Schistosoma japonicum infection from tissues and serum of Microtus fortis,the heart,liver,spleen,lung,kidney,muscle,skin,bone marrow,brain tissue and serum protein of Microtus fortis were collected respectively.The same proteins obtained from rabbits,cattle and mice infected with Schistosoma japonicum as control.The individual soluble proteins were separated by SDS-PAGE and stained with kaomasi blue R-250.The band sizes and their differences were analyzed.Results showed that the 85 ku proteins fractions in serum was more obviously and highly expressed after 12,45 days post-infection in mice than in Microtus fortis;the 135 ku proteins fractions in liver and serum of Microtus fortis was characterized and more highly expressed than other animals.It maybe an important effective molecule against Schistosoma japonicum infection in Microtus fortis.